Transcription and RNA processing — practice questions

Question 1 · difficulty L1 · recall

Removal of introns from eukaryotic primary RNA transcript:

• A. splicing
• B. replication
• C. transduction
• D. translation

Question 2 · difficulty L1 · recall

The RNA carrying codons from DNA to ribosome:

• A. rRNA
• B. tRNA
• C. mRNA
• D. snRNA only

Question 3 · difficulty L2 · understanding

Given below are two statements: Statement I: In the RNA world, RNA is considered the first genetic material evolved to carry out essential life processes. RNA acts as a genetic material and also as a catalyst for some important biochemical reactions in living systems. Being reactive, RNA is unstable. Statement II: DNA evolved from RNA and is a more stable genetic material. Its double helical strands being complementary, resist changes by evolving repairing mechanism. In the light of the above statements, choose the most appropriate answer from the options given below :

• A. Statement I is correct but statement II is incorrect
• B. Statement I is incorrect but statement II is correct
• C. Both statement I and statement II are correct
• D. Both statement I and statement II are incorrect

Question 4 · difficulty L2 · understanding

Given below are two statements : Statement 1 : Transfer RNAs and ribosomal RNA do not interact with mRNA. Statement II : RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence. In the light of the above statements, choose the most appropriate answer from the options given below :

• A. Statement I is correct but statement II is incorrect
• B. Statement I is incorrect but statement II is correct
• C. Both statement I and statement II are correct
• D. Both statement I and statement II are incorrect

Question 5 · difficulty L2 · understanding

Which factor is important for termination of transcription?

• A. $\rho$ (rho)
• B. $\gamma$ (gamma)
• C. $\alpha$ (alpha)
• D. $\sigma$ (sigma)

Question 6 · difficulty L3 · understanding

Which of the following statements are correct with reference to a transcription unit? A. A transcription unit in DNA is defined primarily by three regions : promoter, structural gene and terminator. B. The promoter is said to be located towards the 5'-end of the structural gene. C. The promoter is a DNA sequence that provides binding site for RNA polymerase. D. The promoter defines the template and coding strands. E. The terminator is located towards the $3^{\prime}$-end of the coding strand and it defines the end of the process of transcription. Choose the correct answer from the options given below:

• A. A, B, C and D only
• B. A, C, D and E only
• C. B, C, D and E only
• D. A, B, C, D and E

Question 7 · difficulty L3 · understanding

Which of the following are the post-transcriptional events in an eukaryotic cell? A. Transport of pre-mRNA to cytoplasm prior to splicing. B. Removal of introns and joining of exons. C. Addition of methyl group at 5' end of hnRNA. D. Addition of adenine residues at 3' end of hnRNA. E. Base pairing of two complementary RNAs. Choose the correct answer from the options given below :

• A. B, C, E only
• B. C, D, E only
• C. A, B, C only
• D. B, C, D only

Question 8 · difficulty L3 · understanding

Given below are two statements regarding RNA polymerase in prokaryotes. Statement I : In prokaryotes, RNA polymerase is capable of catalysing the process of elongation during transcription. Statement II : RNA polymerase associate transiently with ‘Rho’ factor to initiate transcription. In the light of the above statements, choose the correct answer from the options given below :

• A. Statement I is true but Statement II is false
• B. Statement I is false but Statement II is true
• C. Both Statement I and Statement II are true
• D. Both Statement I and Statement II are false

Question 9 · difficulty L3 · understanding

Given below are two statements: Statement I: In eukaryotes there are three RNA polymerases in the nucleus in addition to the RNA polymerase found in the organelles. Statement II: All the three RNA polymerases in eukaryotic nucleus have different roles. In the light of the above statements, choose the correct answer from the options given below:

• A. Statement I is correct but Statement II is incorrect
• B. Statement I is incorrect but Statement II is correct
• C. Both Statement I and Statement II are correct
• D. Both Statement I and Statement II are incorrect

Question 10 · difficulty L3 · understanding

Given below are two statements : Statement I : RNA interference takes place in all Eukaryotic organisms as method of cellular defense. Statement II : RNAi involves the silencing of a specific mRNA due to a complementary single-stranded RNA molecule that binds and prevents translation of mRNA In the light of the above statements, choose the correct answer from the options given below.

• A. Statement I is true but Statement II is false.
• B. Statement I is false but Statement II is true.
• C. Both Statement I and Statement II are true.
• D. Both Statement I and Statement II are false.