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NEET-UG Biology

Human Genome Project and DNA fingerprinting — practice questions

8 questions in the bank on this idea. Below are 8 of them, exactly as they appear in a test.

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  1. Question 1 · difficulty L1 · recall

    A major aim of the Human Genome Project:

    • A. cloning every human
    • B. producing only recombinant insulin
    • C. eliminating mutations
    • D. determining the sequence and identifying genes in the human genome

  2. Question 2 · difficulty L1 · recall

    The highly polymorphic repeated sequences classically used in DNA fingerprinting:

    • A. telomerase proteins
    • B. histone genes only
    • C. VNTRs
    • D. ribosomal RNAs

  3. Question 3 · difficulty L2 · understanding

    What was a central goal of the Human Genome Project?

    • A. To replace all human genes with synthetic DNA
    • B. To clone every human gene into bacteria
    • C. Sequence the human genome and identify genes
    • D. To sequence only protein-coding regions

  4. Question 4 · difficulty L3 · understanding

    Arrange the following steps of DNA fingerprinting in a correct sequence. A. Isolation of DNA and its digestion by restriction endonucleases. B. Hybridisation using a labelled VNTR probe. C. Transferring of separated DNA fragments to synthetic membranes. D. Detection of hybridised DNA fragments by autoradiography. E. Separation of DNA fragments by electrophoresis. Choose the correct answer from the options given below :

    • A. A,B,D,C,E\mathrm{A}, \mathrm{B}, \mathrm{D}, \mathrm{C}, \mathrm{E}
    • B. A,D,B,E,C\mathrm{A}, \mathrm{D}, \mathrm{B}, \mathrm{E}, \mathrm{C}
    • C. A,E,C,B,DA, E, C, B, D
    • D. A,E,B,C,D\mathrm{A}, \mathrm{E}, \mathrm{B}, \mathrm{C}, \mathrm{D}

  5. Question 5 · difficulty L3 · understanding

    DNA polymorphism forms the basis of:

    • A. Genetic mapping
    • B. DNA finger printing
    • C. Both genetic mapping and DNA finger printing
    • D. Translation

  6. Question 6 · difficulty L3 · understanding

    Transposons can be used during which one of the following?

    • A. Polymerase Chain Reaction
    • B. Gene Silencing
    • C. Autoradiography
    • D. Gene sequencing

  7. Question 7 · difficulty L3 · understanding

    DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as

    • A. Polymorphic DNA
    • B. Satellite DNA
    • C. Repetitive DNA
    • D. Single nucleotides

  8. Question 8 · difficulty L4 · analysis

    Why are highly variable repetitive DNA regions useful in DNA fingerprinting?

    • A. They are identical in all humans
    • B. They vary among individuals
    • C. They occur only in mitochondrial DNA
    • D. They are never inherited

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