Cloning vectors and their features — practice questions

Question 1 · difficulty L2 · understanding

Silencing of specific mRNA is possible via RNAi because of

• A. Complementary tRNA
• B. Non-complementary ssRNA
• C. Complementary dsRNA
• D. Inhibitory ssRNA

Question 2 · difficulty L3 · understanding

$$\text { Match List I with List II: }$$ $$\text { List-I }$$ $$\text { List-II }$$ A. Genetically modified organism I. Agrobacterium tumefaciens B. Thermostable DNA polymerase II. Bt cotton C. Ti plasmid III. Thermus aquaticus D. pBR322 IV. Escherichia coli

• A. A-II, B-III, C-I, D-IV
• B. A-II, B-I, C-IV, D-III
• C. A-I, B-IV, C-III, D-II
• D. A-I, B-II, C-IV, D-III

Question 3 · difficulty L3 · understanding

Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards:

• A. Ampicillin and tetracycline
• B. Ampicillin
• C. Tetracycline
• D. Gentamycin

Question 4 · difficulty L3 · understanding

In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

• A. White color colonies will be selected.
• B. Blue color colonies grown on ampicillin plates can be selected.
• C. Using ampicillin & tetracycline containing medium plate.
• D. Blue color colonies will be selected.

Question 5 · difficulty L3 · understanding

The blue and white selectable markers have been developed which differentiate recombinant colonies from nonrecombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below:

• A. Statement I is correct but Statement II is incorrect
• B. Statement I is incorrect but Statement II is correct
• C. Both Statement I and Statement II are correct
• D. Both Statement I and Statement II are incorrect

Question 6 · difficulty L3 · understanding

The Bt toxin in genetically engineered Bt cotton kills the pest by:

• A. Creating pores in the midgut
• B. Damaging the respiratory system
• C. Degenerating the nervous system
• D. Altering the pH of body fluids

Question 7 · difficulty L3 · understanding

The "Ti plasmid" of Agrobacterium tumefaciens stands for

• A. Tumour inhibiting plasmid
• B. Tumor independent plasmid
• C. Tumor inducing plasmid
• D. Temperature independent plasmid

Question 8 · difficulty L3 · understanding

Ligation of foreign DNA at which of the following site will result in loss of tetracyclin resistance of pBR322 :

• A. Pst I
• B. Pvu I
• C. EcoR I
• D. BamH I

Question 9 · difficulty L3 · understanding

Which of the following is not a cloning vector?

• A. YAC
• B. pBR322
• C. Probe
• D. BAC

Question 10 · difficulty L3 · understanding

Match List-I with List-II : List - I List - II (a) Gene gun (i) Replacement of a faulty gene by a normal healthy gene (b) Gene therapy (ii) Used for transfer of gene (c) Gene cloning (iii) Total DNA in the cells of an organism (d) Genome (iv) To obtain identical copies of a particular DNA molecule Choose the correct answer from the options given below:

• A. (a) - (ii), (b) - (iii), (c) - (iv), (d) - (i)
• B. (a) - (ii), (b) - (i), (c) - (iv), (d) - (iii)
• C. (a) - (i), (b) - (iii), (c) - (ii), (d) - (iv)
• D. (a) - (iv), (b) - (i), (c) - (iii), (d) - (ii)