Bioreactors and downstream processing — practice questions

Question 1 · difficulty L1 · recall

What is the main purpose of a bioreactor?

• A. To separate DNA only by size
• B. To sequence DNA automatically
• C. Controlled large-scale biological production
• D. Storage of dry chemicals and purified products without any living culture

Question 2 · difficulty L2 · understanding

Why is stirring important in many stirred-tank bioreactors?

• A. It destroys the desired product
• B. It prevents all cell growth
• C. It replaces all nutrients and eliminates the need to monitor dissolved oxygen
• D. It distributes nutrients and oxygen

Question 3 · difficulty L2 · understanding

Identify the part of a bio-reactor which is used as a foam braker from the given figure.

• A. D
• B. c
• C. A
• D. B

Question 4 · difficulty L3 · application

A culture in a bioreactor develops zones with low oxygen and poor nutrient availability. Which operating feature is most directly inadequate?

• A. Mixing and aeration
• B. Automated DNA sequencing of every cell in the production culture
• C. Gel staining
• D. Primer design

Question 5 · difficulty L3 · application

Which stage begins after the desired biological product has been produced in a bioreactor?

• A. DNA replication
• B. Downstream processing
• C. Transformation
• D. Transcription initiation

Question 6 · difficulty L3 · understanding

Given below are two statements: Statement I : The Indian Government has set up GEAC, which will make decisions regarding the validity of GM research. Statement II : Biopiracy is the term used to refer to the use of bio-resources by native people. In the light of the above statements, choose the correct answer from the options given below :

• A. Statement I is true but Statement II is false
• B. Statement I is false but Statement II is true
• C. Both Statement I and Statement II are true
• D. Both Statement I and Statement II are false

Question 7 · difficulty L3 · understanding

Match List-I with List - II : List - I List - II (A) Genetically engineered Human Insulin (I) Gene therapy (B) GM Cotton (II) E. coli (C) ADA Deficiency (III) Antigen-antibody interaction (D) ELISA (IV) Bacillus thuringiensis Choose the correct answer from the options given below:

• A. A-III, B-II, C-IV, D-I
• B. A-II, B-I, C-IV, D-III
• C. A-IV, B-III, C-I, D-II
• D. A-II, B-IV, C-I, D-III

Question 8 · difficulty L5 · analysis

A recombinant protein is produced successfully but contains cellular debris and unwanted proteins. What is the most appropriate next step?

• A. Increase mutation rate
• B. Repeat host-cell transformation even though product synthesis was already successful
• C. Purify it during downstream processing
• D. Remove the recombinant gene