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RNA interference and nematode resistance — practice questions

7 questions in the bank on this idea. Below are 7 of them, exactly as they appear in a test.

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  1. Question 1 · difficulty L1 · recall

    What does RNA interference primarily do?

    • A. It increases translation of every mRNA
    • B. It changes the genetic code globally
    • C. It converts RNA into protein directly
    • D. It silences a matching RNA target

  2. Question 2 · difficulty L2 · understanding

    Why is double-stranded RNA important in RNA interference?

    • A. It triggers silencing of complementary RNA
    • B. It acts as a ribosomal protein
    • C. It permanently removes all DNA
    • D. It converts the target gene into a plasmid

  3. Question 3 · difficulty L2 · understanding

    In the NCERT example of nematode-resistant tobacco, which organism is the target pest?

    • A. Bacillus thuringiensis
    • B. Meloidogyne incognita
    • C. Plasmodium vivax
    • D. Agrobacterium tumefaciens

  4. Question 4 · difficulty L3 · application

    A plant produces RNA complementary to an essential nematode mRNA. What is the expected direct effect in the nematode?

    • A. The nematode DNA is converted into plant DNA
    • B. Every nematode gene is activated
    • C. The matching nematode gene is silenced
    • D. The plant loses all its own mRNA

  5. Question 5 · difficulty L3 · application

    Why is RNAi described as sequence-specific?

    • A. The process works only in specialised bacterial cells that alone contain RNA
    • B. Only one cell type contains RNA
    • C. All RNAs have identical sequences
    • D. Base pairing directs silencing to matching RNA

  6. Question 6 · difficulty L3 · application

    A nematode gene changes enough that its mRNA is no longer complementary to the RNAi trigger produced by a crop. What is most likely?

    • A. The plant must stop photosynthesis
    • B. Silencing efficiency may decrease
    • C. All nematode genes become silent
    • D. The crop automatically produces a new matching RNA

  7. Question 7 · difficulty L4 · analysis

    A transgenic plant expresses both sense and antisense RNA from a nematode gene sequence. What useful product can form?

    • A. Double-stranded RNA
    • B. A new chromosome pair
    • C. A Bt crystal protein automatically
    • D. A bacterial plasmid without replication

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